A few plasmids (pGL1124, pGL1224 and pGL1217) had been built to allow the substitution of 1 allele of CYC9Lister 427 cells. Trypanosomes had been transfected with Just about every plasmid separately, and after that subjected into a next transfection with among the other plasmids, with a special resistance gene, with all pairwise mixtures done., et
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